Simultaneous Gas-liquid Chromatographic Determination of Aldoses and Alduronic Acids

Aldoses and alduronic acids can be simultaneously identified and quantitated by wide-bore capillary gas-liquid chromatography. The aldoses are converted to alditol acetates and the alduronic acids are converted to their corresponding N-propylaldonamide acetates. Standard mixtures containing as little as 100 ng each of arabinose, xylose, mannose, galactose, glucose, inositol, mannuronic, glucuronic, and galacturonic acids can be readily analyzed. Inositol or phenyl-fi-D-glucopyranoside can be used as internal standards.